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Chemical crosslinkers enhance detection of receptor interactomes

Show simple item record Corgiat, Brian A. Nordman, Jacob C. Kabbani, Nadine 2015-10-12T17:28:05Z 2015-10-12T17:28:05Z 2014-01-07
dc.identifier.citation Corgiat BA, Nordman JC and Kabbani N (2014) Chemical crosslinkers enhance detection of receptor interactomes. Front. Pharmacol. 4:171. doi: 10.3389/fphar.2013.00171 en_US
dc.description.abstract Receptor function is dependent on interaction with various intracellular proteins that ensure the localization and signaling of the receptor. While a number of approaches have been optimized for the isolation, purification, and proteomic characterization of receptor–protein interaction networks (interactomes) in cells, the capture of receptor interactomes and their dynamic properties remains a challenge. In particular, the study of interactome components that bind to the receptor with low affinity or can rapidly dissociate from the macromolecular complex is difficult. Here we describe how chemical crosslinking (CC) can aid in the isolation and proteomic analysis of receptor–protein interactions. The addition of CC to standard affinity purification and mass spectrometry protocols boosts the power of protein capture within the proteomic assay and enables the identification of specific binding partners under various cellular and receptor states. The utility of CC in receptor interactome studies is highlighted for the nicotinic acetylcholine receptor as well as several other receptor types. A better understanding of receptors and their interactions with proteins spearheads molecular biology, informs an integral part of bench medicine which helps in drug development, drug action, and understanding the pathophysiology of disease.
dc.description.sponsorship This work was supported by a Wings for Life Spinal Cord Research Grant to Nadine Kabbani. Publication of this article was funded in part by the George Mason University Libraries Open Access Publishing Fund. en_US
dc.language.iso en_US en_US
dc.publisher Frontiers Media en_US
dc.rights Attribution 3.0 United States *
dc.rights.uri *
dc.subject nicotinic acetylcholine receptor en_US
dc.subject chemical crosslinking en_US
dc.subject mass spectrometry en_US
dc.subject protein–protein interaction en_US
dc.subject signaling network en_US
dc.subject interactome en_US
dc.title Chemical crosslinkers enhance detection of receptor interactomes en_US
dc.type Article en_US

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